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KMID : 0376219780150010021
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Chonnam Medical Journal
1978 Volume.15 No. 1 p.21 ~ p.27
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Antibiotic Resistance Transfer of Pseudomonas aeruginosa
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Abstract
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Pseudomonas aerugionsa(Ps..) has rapidly been replacing Staphylococcus aureus as a nosocomial agent. Investigation on the behaviour of this organism´s drugresistance transfer may, therefore, be urgently needed in order to develop a way to. contain its menace in medicine.
Ps. AH 9, which was lysed completely by phage AH 9 and showed resistances to all test drugs except for colistine and polymyxin B, was used as a donor strain. P s. AH 1, Ps. AH 5, and Ps. AH 7, which were not lysed by the phage and susceptible to a few more antibiotics, were chosen as recipients among seventy odd isolates. E. coli ML 1410, E. coli K-12x407, and S. typhi H 901-4 were also submitted as intergeneric recipients.
Intrageneric transfer of resistance from the donar to recipients, Ps. AH I and Ps. AH 7, was observed in moderate frequencies in all agents tested (10-2_ 10-3) Carb. resistance transfer, however, did not occur in recipient Ps. AH 5, which was originally resistant to both GM and KM.
Intergeneric transfer of resistance was extremely difficult. Mixed culture of the donor and recipient E. coli ML 1410 did not bring about any conversion in the, recipient strain, while only a trace transfer was observed in some resistances between the Ps. AH 9 and recipients E. coli K-12 x 407 and S. typhi H 901-4 (10-7,10-8).
Disimilarity in DNA between the donor intergeneric recipient strains may account for the unacceptability of resistance transfer.
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